Coding

Part:BBa_K1418020

Designed by: Dallin Christensen   Group: iGEM14_Utah_State   (2014-10-08)

amylase

alpha-amylase

This part is the RFC 23 compatible malS coding sequence encoding E.coli alpha amylase. The native ribosome binding site and the predicted cleavage signal peptide have been removed from BBa_K523001.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 336
    Illegal AgeI site found at 1501
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None